Morphological and cytochemical characterization of the peripheral blood cells of farmed streaked prochilod Prochilodus lineatus (Characiformes, Prochilodontidae)

ABSTRACT Morphological and cytochemical studies of peripheral blood cells of fish have improved the understanding of their functions and cell types. The present study performed the Morphological and cytochemical analysis of the peripheral blood of Prochilodus lineatus, Characiform native to South America, which has been gaining space in local aquaculture and as a species introduced in Asia. Our analysis provided information on the morphological and cytochemical characteristics of the leukocytes, for the formulation of hypothesis about their role in the immune system of the species. It was found that Prochilodus lineatus has morphological and cytochemical features in common with other fish species, mainly of the Characiformes order. However, we detected the presence of heterophils and PAS positive granulocytes simultaneously with neutrophils. We also found that heterophils and PAS positive granulocytes are very similar, both morphologically and cytochemically.

摘要
鱼类外周血细胞的形态学与细胞化学研究，有助于深化对其功能及细胞类型的认知。本研究针对南美本土脂鲤目（Characiformes）物种双线脂鲤（Prochilodus lineatus）的外周血开展形态学与细胞化学分析；该物种在当地水产养殖业中的占比持续提升，同时也作为引入物种在亚洲地区得到推广应用。本研究的分析结果提供了该物种白细胞的形态学与细胞化学特征相关数据，为阐释其在该物种免疫系统中的作用提供了假说依据。研究发现，双线脂鲤的形态学与细胞化学特征与其他脂鲤目鱼类存在诸多共性。但本研究同时检测到异嗜性粒细胞（heterophils）、过碘酸-雪夫染色（PAS）阳性粒细胞与中性粒细胞共存的现象。此外，本研究还证实，异嗜性粒细胞与PAS阳性粒细胞在形态学与细胞化学层面均高度相似。