Whole-cell recording parameters from ‘higher’ CA1 and striatal neurons (n = 36) compared to brainstem neurons (n = 65) responding to 10 minutes of oxygen/glucose deprivation.
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All neurons were exposed to 10 minutes OGD except 16 of the 31 dNTS neurons which underwent 15 minutes of OGD. Post-OGD measurements made 15–30 minutes following return to control aCSF. More details of the electrophysiological properties of each neuron as well as the properties of newly acquired brainstem neurons recorded in post-OGD slices can be found at http://hdl.handle.net/1974/7578[36]. Abbreviations: Rmp, resting membrane potential; Max Depol., maximum depolarization of anoxic depolarization; AP Ampl., action potential amplitude; Rin, whole-cell input resistance. % Rmp recovery was calculated before correcting for a +14 mV junction potential.
所有神经元均接受10分钟氧糖剥夺(Oxygen-Glucose Deprivation, OGD)处理,仅31个背侧孤束核(dorsal nucleus of the solitary tract, dNTS)神经元中有16个接受了15分钟的OGD处理。OGD后的检测于恢复至对照人工脑脊液(artificial cerebrospinal fluid, aCSF)后的15–30分钟内完成。关于每一例神经元的电生理特性,以及在OGD后脑片记录的新获取脑干神经元的特性的更多细节,可参见http://hdl.handle.net/1974/7578[36]。
缩写说明:Rmp为静息膜电位(resting membrane potential);Max Depol.为缺氧去极化的最大去极化幅度;AP Ampl.为动作电位幅度(action potential amplitude);Rin为全细胞输入电阻。静息膜电位恢复百分比的计算未校正+14 mV的接头电位。
创建时间:
2015-12-02



