Rat IUGR islet transcriptome sequencing at 2 time points

Intrauterine growth restriction (IUGR) increases the risk of developing type 2 diabetes during adulthood. At day 18 of gestation, we used bilatual uterine artery ligation (BUAL) to restrict nutrient supply to developing rat fetuses to produce IUGR pups. At 2 and 10 weeks of age pancreatic islets were isolated and total RNA extracted using Trizol. Samples with RNA integrity numbers greater than 7 were used to generate libraries宫内生长受限（intrauterine growth restriction, IUGR）会提升个体成年后罹患2型糖尿病的风险。本研究于妊娠第18天，通过双侧子宫动脉结扎（bilateral uterine artery ligation, BUAL）限制发育中大鼠胎鼠的营养供应，以此构建宫内生长受限幼鼠模型。分别在幼鼠2周龄与10周龄时分离其胰岛，采用Trizol法提取总RNA；选取RNA完整性数大于7的样本构建测序文库。