Characterization of Anatomical, Macromolecular, and Gene Expression Changes in the Retinal and Retinal Pigmented Epithelium of the nm3342 Mouse: A Potential Model of Serous Retinal Detachment

To characterize gene expression changes in mice following serous retinal detachments, we employed whole genome microarray expression profiling as a tool to identify genes with the potential effects on the neural retina as well as retinal pigmented epithelium. Total RNA was harvested from nm3342 (mutant) and age-matched wild-type (C57Bl/6J) mice at P30 or P365 for examination using microarray analysis. Retinas and retinal pigmented epithelium was harvested from both age-matched mutant and wild-type mice.

为阐明浆液性视网膜脱离后小鼠的基因表达变化特征，本研究采用全基因组微阵列表达谱分析（whole genome microarray expression profiling）技术作为研究工具，旨在筛选可能对神经视网膜及视网膜色素上皮（retinal pigmented epithelium）具有调控作用的基因。我们分别于出生后第30天（P30）与第365天（P365），从nm3342突变型小鼠及年龄匹配的野生型（C57Bl/6J）小鼠体内提取总RNA，用于后续微阵列分析检测。同时从同年龄的突变型与野生型小鼠体内分离获取视网膜与视网膜色素上皮。