Kinship perspective study on subjects with celiac disease with first-degree non-celiac relatives.

One hundred and one participants were recruited and assessed for gut microbiota using third-generation technology (ONT), with the aim of sequencing the almost full ribosomal RNA bacterial operon. Regular host covariates of the gut microbiota (sex, age, BMI), aside from a wide range of other host-associated variables (diet), were used to delimit gut microbiota profiles and recover the differential abundance of more than 200 bacterial species detected in faecal samples.

本研究共招募101名受试者，采用第三代测序技术（ONT）对其肠道菌群进行检测，旨在对近乎完整的细菌核糖体RNA（ribosomal RNA）操纵子进行测序。研究使用性别、年龄、体重指数（BMI）等肠道菌群常见宿主协变量，以及涵盖饮食在内的一系列其他宿主相关变量，用于界定肠道菌群特征，并解析粪便样本中检出的200余种细菌的差异丰度情况。