Expression data from embryonic mouse spinal cords from NFIX knockout and wild-type mice.. Expression data from embryonic mouse spinal cords from NFIX knockout and wild-type mice.

The generation of cellular identity and diversity within the developing spinal cord is critically dependent on networks of gene expression controlled by transcription factors, such as Nuclear Factor One X (NFIX). NFIX has been identified as an important factor in promoting astrocyte formation during embryonic mouse spinal cord development. To gain a more comprehensive understanding of the transcriptional landscape controlled by NFIX within the developing spinal cord, here we performed microarray analysis on E14.5 wild-type and Nfix-/- mouse spinal cords, the age at which the expression of NFIX by neural progenitor cells lining the spinal central canal is strongest. Overall design: Whole spinal cords from Nfix-/- mouse embryos at E14.5 (and wild-type littermate controls; n=4) were dissected for RNA extraction and hybridization on an Affymetrix Mouse Transcriptome Analysis microarray.

发育中的脊髓内细胞身份的建立与多样性的形成，高度依赖于由转录因子（如核因子一X（Nuclear Factor One X，NFIX））所调控的基因表达网络。已有研究证实，NFIX在小鼠胚胎脊髓发育过程中可促进星形胶质细胞的生成，是一类关键调控因子。为更全面地解析发育脊髓中NFIX所调控的转录组全景，本研究对胚胎第14.5天（E14.5）的野生型及Nfix-/-小鼠脊髓开展了基因芯片分析——该时间点正是脊髓中央管内衬神经前体细胞表达NFIX水平最高的时期。实验整体设计：取E14.5天Nfix-/-小鼠胚胎（及同窝野生型对照，每组n=4）的完整脊髓，提取RNA后在Affymetrix小鼠转录组分析微阵列芯片上进行杂交检测。