snATAC-seq of the gray short-tailed opossum cerebellum in two developmental stages. snATAC-seq of the gray short-tailed opossum cerebellum in two developmental stages

This study used droplet-based snATAC-seq to profile the chromatin accessibility landscape of 19,204 nuclei in the opossum (Monodelphis domestical) cerebellum across two developmental stages (postnatal day 21 and adult). The study included two biological replicates per stage, one from each sex, and an additional adult sample enriched for white matter. Cerebelli were dissected in two halves, nuclei were extracted from one half and profiled using 10x single-cell ATAC reagent kit (v1.1) and a Chromium controller. The white matter enriched sample was dissected from coronal cerebellum slices. Libraries were sequenced using paired-reads on Illumina NextSeq 550 and initial data processing was performed using Cellranger ATAC (1.1).

本研究采用基于液滴的单细胞核转座酶可及性测序（droplet-based snATAC-seq），对两个发育阶段（出生后第21天与成年期）的灰短尾负鼠（Monodelphis domestical）小脑中19204个细胞核的染色质可及性图谱进行了表征。本研究每个发育阶段设置两个生物学重复，分别取自不同性别，此外还包含1个富集白质的成年样本。研究人员将小脑标本分为两半，取其中一半提取细胞核，并使用10x单细胞ATAC试剂盒（v1.1）及Chromium控制器（Chromium controller）完成图谱分析。富集白质的样本取自小脑冠状切片。测序文库采用Illumina NextSeq 550平台进行双端测序，初始数据处理通过Cellranger ATAC（1.1）软件完成。