Meis1 function in leukemogenesis

Genome wide ChiP-seq and RNA-seq study determining the global binding sites and target genes of Meis1 during leukemogenesis. Primary hematopoietic cells pre-transformed with HoxA9 were transduced with inducible Meis1 and binding as well as RNA-inventory of the cells were determined by NGS based sequencing.

本研究采用全基因组染色质免疫共沉淀测序（ChIP-seq）与RNA测序（RNA-seq），解析白血病发生过程中Meis1的全局结合位点及其靶基因。将经HoxA9预转化的原代造血细胞以诱导型Meis1进行转导后，通过基于下一代测序（Next-Generation Sequencing，NGS）的技术检测细胞的结合状态与RNA转录谱。