ExtFig7B_SLC7A5_intron_R1-R2-R3_LG290-292-293_annotated.tif

Xrn1 knock out A549 cells were infected with WT A/Puerto Rico/8/1984 (H1N1) ("PR8"), or PA-X deficient PR8-PA(∆X) viruses, or mock infected for 16 hours before RNA extraction. 5’RACE was then performed using primers specific for BCAP31 or SLC7A5 that can differentiate between bands with and without introns. The PCR products were run on an agarose gel.

将Xrn1基因敲除的A549细胞分别以野生型A/波多黎各/8/1984（H1N1）（下称PR8）、PA-X缺陷型PR8-PA(∆X)病毒感染，或进行模拟感染，处理16小时后提取RNA。随后使用针对BCAP31或SLC7A5的特异性引物开展5'末端快速扩增技术（5' Rapid Amplification of cDNA Ends，5'RACE）实验，以区分含有内含子与不含内含子的扩增条带。将PCR扩增产物进行琼脂糖凝胶电泳检测。