SexSib-RadSeq

Sturgeons are ancient fish that are commercially important for caviar production. They are endangered in the wild due to overfishing, river pollution, and human action. Our laboratory works in sex differentiation of fish using the Siberian sturgeon as one of the piscine models. In order to identify a SNP or a series of SNPs associated with the sex we have generated a high-quality >74K SNP panel from female and male Acipenser baerii using a high-depth RAD-seq approach, but no SNPs were found to be associated significantly with the sex using the number of fish sequenced. This SNP panel will facilitate many types of genetic studies, including high-resolution genomic analysis. This data can be used to develop strategies to conserve wild populations and improve aquaculture caviar production.

鲟鱼是一类古老的鱼类，因鱼子酱生产而具备重要商业价值。野生鲟鱼因过度捕捞、河流污染及人类活动而濒临灭绝。本实验室专注于鱼类性别分化研究，其中西伯利亚鲟（Acipenser baerii）为我们选用的鱼类模型之一。为筛选与性别相关的单核苷酸多态性（Single Nucleotide Polymorphism, SNP）位点，本团队采用高深度限制性位点相关DNA测序（RAD-seq）技术对雌雄西伯利亚鲟进行测序，构建了包含超过74K个位点的高质量SNP分型面板；但受限于当前已测序的样本量，未发现与性别显著相关的SNP位点。该SNP分型面板可助力多项遗传研究，包括高分辨率基因组分析。本数据集可用于制定野生种群保护策略，以及提升水产养殖鱼子酱的生产效率。