Core TF Foxc1/2 reinforce differentiation of adult podocyte in glomeruli

We identified Fox1 and Foxc2 core transcription factors in kidney glomeruli. To investigate their transcriptional regulatory roles in glomeruli, we performed ChIPseq of Foxc1 and Foxc2 in adult mouse kidney glomeruli. The genome wide distribution of Foxc1/2 binding sites revealed they regulated the differentiation and mature state of adult podocyte to maintain kidney homeostatis. Mouse kidney glomeruli were isolated by perfusion of magenetic beads and collection with MPC. ChIP-seq was performed with specific antibody.

我们在肾脏肾小球中鉴定出Fox1与Foxc2两类核心转录因子（core transcription factors）。为探究二者在肾小球内的转录调控功能，我们对成年小鼠肾脏肾小球中的Foxc1及Foxc2开展了染色质免疫沉淀测序（ChIP-seq）实验。通过分析Foxc1/2结合位点的全基因组分布特征，我们发现二者可调控成年足细胞（podocyte）的分化与成熟状态，以维持肾脏稳态。本次实验中，我们通过磁珠灌注结合MPC分离得到小鼠肾脏肾小球，并使用特异性抗体完成了ChIP-seq检测。