SLE serum induced transcripts regulated by IFNa2b. Homo sapiens

To explore the full extent of IFN-regulated transcriptional changes, we exposed monocytes from two healthy donors to recombinant type I IFN (IFN-α2b) in vitro. RNA was extracted at 6 hrs and the expression data was normalized to that of monocytes cultured with medium. Overall design: Blood monocytes isolated from healthy volunteers were incubated with 20% autologous serum alone or in the presence of 1000 U/ml of IFNα2b (Schering Plough, Kenilworth, NJ) in 6-well plates at a concentration of 106 monocytes per well in 3 ml of media. After incubation for six hours at 37 ⁰C, cells were harvested and RNA was extracted.

为全面探究干扰素（IFN）调控的转录改变的全貌，我们将两名健康供者的单核细胞在体外暴露于重组I型干扰素（IFN-α2b）中，于培养6小时后提取RNA，并将表达数据与仅以培养基培养的单核细胞的表达数据进行标准化处理。实验整体设计：从健康志愿者体内分离的血液单核细胞，以每孔1×10^6个细胞、3ml培养基的密度接种于6孔板中，分为两组：一组仅添加20%自体血清培养，另一组同时添加1000 U/ml的IFNα2b（先灵葆雅公司，美国新泽西州肯尼沃斯市）培养。于37℃孵育6小时后收集细胞并提取RNA。