Morphometric data and locomotor and social behavior data of male Japanese quail during in a Social Interaction test, in birds reared in an enriched or a standard environment.

Environmental enrichment (EE) protocol
During the EE in the brood boxes (8-28 days old), 3 different sets of enrichments were rotated between boxes every 7 days. After reallocation in home cages at 28 days of age, EE was applied in 3 periods with different enrichment sets. Within each period, EE home cages received weekly one of the three enrichment sets in a random order in such a manner that all EE animals were exposed to the 3 enrichment sets. The replacement of the enrichment items was done on a fixed day and time every week, and during this procedure all the animals were removed from the box and placed in a basket. Control animals underwent the same manipulation procedure.

Social Interaction Test was performed between 118 and 130 days of age. This test is described in detail in Caliva et al (2017, Pout. Sci) including a figure with the schematic representation of the test procedure. Briefly, the SI test consists in encounters between an unfamiliar test adult male and a PC stimulus adult male, in the presence of the test bird’s female cagemate (audience). First, the test male and it’s female cagemate were placed in a central compartment separated by an opaque partition from a PC stimulus male. After 2 min, the test male and the PC stimulus male, remained in the same compartment, while the female cagemate was placed in a nearby compartment at one side of the apparatus, and used as a social audience. Immediately after, the central opaque partition was removed and the test and PC stimulus birds were allowed to interact. Direct interaction lasted a maximum of 10 min. However, if during the interaction a quail received more than 5 consecutive aggressive pecks, showed a clear and continued escaping (retrieval) behavior, and/or showed any sign of physical damage, the interaction was immediately interrupted. A video-camera was positioned 1 m above the apparatus and connected to a computer that allowed constant monitoring and recording during the test while out of the sight of the birds. Using ANY-maze the following aggressive behaviors were recorded: pecks, grabs, mounts, cloacal contacts, threats, chase, and attack with claws. Herein, when grabs, mounts or cloacal contacts were performed by one male towards another male, they were considered as aggressive behaviors. Males that performed more than 5 aggressiveness behaviors were considered aggressive (Ag), and males that did not perform any aggressive behavior towards PC opponent were considered non-aggressive (nonAg).
Males exposed to the experimental apparatus without the presence of an opponent (testCON) and non-manipulated males that always remained in the home cage (Naive) with their cagemate were used as control groups.

环境富集（Environmental Enrichment, EE）实验方案
在8~28日龄的育雏笼环境富集实验期间，每7天对各笼箱轮换3组不同的富集装置。待动物于28日龄转移至居家笼后，EE实验分为3个阶段，每个阶段采用不同的富集组合。各阶段内，每周随机选取3组富集装置中的一组安置于EE组居家笼中，确保所有EE组动物均能接触全部3组富集装置。富集物品的更换于每周固定日期和时刻进行，操作期间需将所有动物移出笼箱并转移至转运篮中。对照组动物需接受完全一致的操作流程。

社交互动测试（Social Interaction Test, SI Test）实施于118~130日龄期间。该测试的详细方案已发表于Caliva等人2017年《家禽科学》（Poultry Science, Pout. Sci）的研究中，文中附有测试流程的示意图。简言之，SI测试为陌生的测试成年雄鸟与刺激成年雄鸟（PC stimulus adult male）在测试雄鸟的雌性笼养同伴（社交观众）在场时的互动实验。具体流程如下：首先将测试雄鸟及其雌性笼养同伴放置于中央隔间，该隔间与刺激雄鸟所在隔间以不透明隔板分隔。2分钟后，测试雄鸟与刺激雄鸟仍留于原中央隔间，而雌性笼养同伴被转移至装置一侧的邻近隔间，作为社交观众。随后立即移除中央的不透明隔板，允许测试雄鸟与刺激雄鸟进行互动。直接互动时长最长为10分钟。但若互动过程中某只鹌鹑连续遭受超过5次攻击性啄击、出现持续明确的逃避（逃窜）行为，或出现任何躯体损伤迹象，则需立即终止互动。

于装置上方1米处架设一台摄像机，连接至计算机以在测试期间持续监控并录制行为，同时确保摄像机处于鸟类的视野之外。使用ANY-maze软件记录以下攻击性行为：啄击、抓握、爬跨、泄殖腔接触、威吓、追逐及爪击。本研究中，若某雄鸟对另一雄鸟实施抓握、爬跨或泄殖腔接触行为，则将其判定为攻击性行为。实施超过5次攻击性行为的雄鸟被归类为攻击性个体（Ag），未对刺激雄鸟实施任何攻击性行为的雄鸟则被归类为非攻击性个体（nonAg）。

将仅暴露于实验装置但无对手存在的雄鸟（testCON，测试对照组），以及始终留在居家笼中、未接受任何操作的雄鸟（Naive，空白对照组）与其笼养同伴共同作为对照组。