Human colon epithelial cells treated with Clostridium difficile Toxins A and B

Toxin A and B from Clostridium difficile are the primary virulence factors in Clostridium difficile disease. The changes in gene transcription of human colon epithelial cells were investigated in vitro in order to better understand the many effects of both toxins. HCT-8 cells were treated with 100 ng/ml of either Toxin A or B (TcdA or TcdB). RNA was isolated 2, 6, and 24 hours after addition of toxin from untreated and toxin-treated cells.

艰难梭菌（Clostridium difficile）产生的毒素A与毒素B是该菌致病的主要毒力因子。为深入解析这两种毒素的多重生物学效应，本研究通过体外实验探究了人结肠上皮细胞的基因转录变化。实验以100 ng/ml的毒素A或毒素B（TcdA或TcdB）处理HCT-8细胞，并分别于毒素添加后的2小时、6小时及24小时，从未处理细胞与毒素处理细胞中分离RNA。