Zea mays Transcriptome or Gene expression. Zea mays

affy_zeawall_ril_maize - Four major QTL explaining lignin content and cell wall degradability variation have been identified in the F288 x F271 RIL progeny (Roussel et al., 2002, Maydica 47: 9-20). Gene expression has been considered as a way in identification of candidate gene underlying QTL. Five lines chosen for their allelic pattern at four main QTL were considered for expression studies, two lines with all four favorable alleles (RIL99, RIL54), two lines with the unfavorable allele at bin 6.06 QTL position (RIL39 , RIL71), and one line with the unfavorable allele at bin 9.02 QTL position (RIL118). Expression study will be investigated in ear internode at two stages emerging tassel and early silking stages with two replicates. Plants were cropped at INRA Lusignan (Vienne, France) in 2009 in a bloc design with two four-row replicates. Below-ear internodes of five representative RIL plants of one row in each replicate were harvested at the two stages and pooled. Keywords: genotype comparison, time course Overall design: 20 arrays - maize

affy_zeawall_ril_maize数据集——本研究在F288与F271杂交得到的重组自交系（Recombinant Inbred Line, RIL）后代群体中，已鉴定出4个可解释木质素含量与细胞壁降解率变异的主效数量性状基因座（Quantitative Trait Locus, QTL）（Roussel等，2002，Maydica 47: 9-20）。基因表达分析已被用作鉴定QTL区域内候选基因的手段。本研究选取了在4个主要QTL位点上具有特征性等位基因模式的5个玉米株系开展表达分析：其中2个株系携带全部4个有利等位基因（RIL99、RIL54），2个株系在bin 6.06的QTL位点携带不利等位基因（RIL39、RIL71），剩余1个株系在bin 9.02的QTL位点携带不利等位基因（RIL118）。将分别在抽雄期和吐丝早期两个发育阶段，对上述株系的玉米穗下节间进行表达量检测，每个设置两次重复。供试材料于2009年在法国维埃纳省吕尼南的法国国家农业研究院（INRA）试验田种植，采用区组设计，设置2个四行区重复。在每个重复的小区中选取5株具有代表性的RIL株系，分别在上述两个发育阶段采集其穗下节间组织并混合。关键词：基因型比较、时间进程；整体实验设计：20张芯片——玉米