Global 5mC and 5hmC levels in sixteen pairs of HCC and non-HCC samples and two HCC cell lines investigated by UPLC-MS/MS

We performed UPLC-MS/MS to investigate global 5mC and 5hmC levels in sixteen pairs of HCC and non-HCC samples and two HCC cell lines (97L and LM6 cells). We found that 5mC and 5hmC were frequently decreased in 71% and 100% of the HCC specimens, respectively, compared with non-HCC specimens. Correspondingly, 5mC and 5hmC levels in the two HCC cell lines remained relatively low.

本研究采用超高效液相色谱-串联质谱（Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry，UPLC-MS/MS）技术，对16对肝细胞癌（Hepatocellular Carcinoma，HCC）与配对非肝细胞癌样本，以及2株肝细胞癌细胞系（97L细胞、LM6细胞）中5-甲基胞嘧啶（5-methylcytosine，5mC）和5-羟甲基胞嘧啶（5-hydroxymethylcytosine，5hmC）的全局水平开展了检测分析。结果显示，与非肝细胞癌样本相比，71%的肝细胞癌组织标本中5mC水平普遍降低，100%的肝细胞癌组织标本中5hmC水平均出现显著下调；与之对应，2株肝细胞癌细胞系中的5mC与5hmC水平同样维持在相对较低的水平。