Human fetal penile smooth muscle cells (hfPSCM): Control vs PCB mixtures treatments. Homo sapiens

The effects exerted by three mixtures of Polychlorinated Biphenyls (PCB), one featuring dioxin-like (DL) and two featuring non dioxin-like (NDL) congeners, on human fetal corpora cavernosa cells representative of a major male endocrine-sensitive organ, have been evaluated by gene expression profiling. PCB congeners concentrations used were derived from human internal exposure data to explore the impact of the adult body burden on a fetal tissue. Cell exposure were performed for 72h mimicking a chronic exposure. Overall design: Experiment conditions: control, three treatments with PCB-Mix1, PCB-Mix2 or PCB-Mix3. Biological replicates: 3 control replicates, 3 replicates per treatment. Technical replica: 2 (dye swap) per biological replica. Direct comparison design (control vs treated samples) in 12/16 arrays (biological replicates 1 and 2); Loop design in 4/16 arrays (biological replicate 3).

本研究通过基因表达谱分析，评估了三种多氯联苯（Polychlorinated Biphenyls, PCB）混合物对人类胎儿阴茎海绵体细胞的影响——该细胞作为男性主要内分泌敏感器官的代表；三种混合物中，1种以类二噁英型（dioxin-like, DL）同系物为特征，另外2种以非类二噁英型（non dioxin-like, NDL）同系物为特征。本次实验所用的PCB同系物浓度取自人体内暴露数据，旨在探究成年个体体内负荷对胎儿组织的影响。细胞暴露时长设置为72小时，以模拟慢性暴露过程。实验整体设计如下：实验分组包括对照组，以及分别经PCB-Mix1、PCB-Mix2、PCB-Mix3处理的三个实验组。生物学重复：对照组设置3次生物学重复，每个实验组各设置3次生物学重复。技术重复：每个生物学重复设置2次技术重复，采用染料互换法。在16张基因芯片中，12张采用直接比较设计（对照组与处理组样本对比），对应生物学重复1和2；剩余4张采用环路设计，对应生物学重复3。