Transcriptome profiling of liver samples from wild-type and Adamts12-/- mice in response to CCl4-induced liver injury.

We used Adamts12−/− and wild-type mice generated and genotyped by El Hour et al. 2010 (PMID: 20208563). eight-week-old females were treated by intraperitoneal injections of CCl4 (Sigma-Aldrich, St. Louis, MO, USA) diluted at 3% v/v in olive oil. A single dose of CCl4 0.3 ml/kg of mouse body weight was administered (acute treatment) and mice were sacrificed after 4h, 12h, 24h or 7 days. Control mice were treated with the vehicle (olive oil). Liver samples were collected, weighed and treated as previously described (Kesteloot et al. 2007, PMID: 17929299). We performed gene expression profiling analysis using data obtained from liver samples of wild-type or Adamts12_KO mice at different time points after CCl4 (or vehicle) injection. Comparative gene expression profiling analysis using data obtained from liver samples of wild-type or Adamts12_KO mice at 4h, 12h, 24h or 7 days after CCl4 or vehicle injection.

本研究使用了El Hour等人2010年构建并完成基因型鉴定的Adamts12基因敲除（Adamts12−/−）与野生型（wild-type）小鼠，相关文献编号为PMID: 20208563。选取8周龄雌性小鼠，采用腹腔注射方式给药：将四氯化碳（CCl4，Sigma-Aldrich，美国密苏里州圣路易斯市）以3%体积比稀释于橄榄油中，单次给药剂量为0.3 ml/kg小鼠体重，用于急性造模；分别于给药后4h、12h、24h及7天处死小鼠。对照组小鼠仅给予等量溶剂橄榄油。收集肝脏样本并称重，按照Kesteloot等人2007年报道的方法进行处理，相关文献编号为PMID: 17929299。本研究针对四氯化碳（或溶剂）注射后不同时间点的野生型或Adamts12基因敲除小鼠的肝脏样本开展基因表达谱分析，并针对四氯化碳或溶剂注射后4h、12h、24h及7天的野生型或Adamts12基因敲除小鼠肝脏样本开展对比基因表达谱分析。