Multi-dimensional histone modifications for coordinated regulation of gene expression under hypoxia [ChIP-Seq]. Homo sapiens

Analysis of epigenetic changes at hypoxia at gene level. The hypothesis was that different types of methylation affect gene expression coordinately regulate specific induction or repression of hypoxia-responsive genes. Overall design: genomic DNA obtained from adipocyte-derived stem cell subjected to 48 hours in hypoxic condition compared to normoxia condition.

缺氧条件下基因水平的表观遗传变化分析。本研究提出的核心假说为：不同类型的甲基化修饰可通过影响基因表达，协同调控缺氧应答基因（hypoxia-responsive genes）的特异性诱导与阻遏。实验设计概况：将经缺氧条件培养48小时的脂肪源性干细胞所提取的基因组DNA，与常氧条件对照组的样本进行对比分析。