Identification of plasmid IncQ1 and NTEKPC-IId harboring bla KPC-2 in isolates from Klebsiella pneumoniae infections in patients from Recife-PE, Brazil

Abstract INTRODUCTION: This study investigated the genetic environment of bla KPC-2 in Klebsiella pnemoniae multi-drug resistant clinical isolates. METHODS: Four carbapenemase gene isolates resistant to carbapenems, collected from infected patients from two hospitals in Brazil, were investigated using polymerase chain reaction and plasmid DNA sequencing. RESULTS: The bla KPC-2 gene was located between ISKpn6 and a resolvase tnpR in the non-Tn4401 element (NTEKPC-IId). It was detected on a plasmid belonging to the IncQ1 group. CONCLUSIONS To our knowledge, this is the first report of the presence of the bla KPC-2 gene in the NTEKPC-IId element carried by plasmid IncQ1 from infections in Brazil.

摘要 引言：本研究针对多重耐药肺炎克雷伯菌（Klebsiella pneumoniae）临床分离株中bla KPC-2基因的遗传环境展开探究。方法：采集自巴西两家医院感染患者体内的4株携带碳青霉烯酶基因且耐碳青霉烯类的分离株，采用聚合酶链反应（polymerase chain reaction, PCR）与质粒DNA测序技术进行分析。结果：bla KPC-2基因位于非Tn4401元件（NTEKPC-IId）中的ISKpn6与解离酶tnpR之间，且该基因存在于IncQ1型质粒中。结论：据我们所知，本研究首次报道了巴西感染病例中，IncQ1型质粒携带的NTEKPC-IId元件内存在bla KPC-2基因。