Evaluation of gene expressions in epididymal white adipose tissue (eWAT) of genetic and diet-induced NASH model.

To investigate the alterlation of eWAT gene expression in diet-induced NASH model with adipose tissue insulin resistance, we evaluated eWAT of control mice and adipocyte-specific PDK1 knockout (PDK1KO) mice fed either normal chow(NC) or Gubra amylin NASH (GAN) diet for 16weeks after weaning at 4 weeks old. We then performed gene expression profiling analysis using data obtained from RNA-seq of these 4 groups. Comparative gene expression profiling analysis of RNA-seq data for eWAT of 4 groups[Control(NC), Contro(GAN), PDK1KO(NC),PDK1KO(GAN)].

为探究伴脂肪组织胰岛素抵抗的饮食诱导非酒精性脂肪性肝炎（NASH）模型中附睾白色脂肪组织（eWAT）的基因表达改变，我们选取4周龄断奶的小鼠，分别饲喂普通饲料（normal chow, NC）或Gubra胰淀素NASH（GAN）饲料，持续干预16周。实验分组包括对照组小鼠与脂肪细胞特异性丙酮酸脱氢酶激酶1（PDK1）敲除（PDK1KO）小鼠，我们采集了上述各组小鼠的附睾白色脂肪组织。随后，我们基于这4组样本的RNA测序（RNA-seq）数据开展基因表达谱分析，并针对4组[对照组(NC)、对照组(GAN)、PDK1KO(NC)、PDK1KO(GAN)]小鼠的附睾白色脂肪组织RNA测序数据进行对比基因表达谱分析。