Ultra-Processed Foods drive Intestinal Barrier permeability increasing Microvascular Disease risk

Diabetic (db/db) or non diabetic (db/h) mice were fed a control, heat treated or heat treated with resistant starch diet for 10 weeks. Cecal microbiota was collected and the V3-V4 region of the 16S rRNA gene was sequenced using primer pair Bac_SSU_341F-806wR.

将糖尿病（db/db）型与非糖尿病（db/h）型小鼠分为三组，分别饲喂对照日粮、热处理日粮及添加抗性淀粉的热处理日粮，持续干预10周。随后采集小鼠盲肠微生物菌群，采用引物对Bac_SSU_341F-806wR对16S rRNA基因的V3-V4可变区进行测序。