A three-dimensional ex vivo model recapitulates in vivo features and unravels increased drug resistance in childhood acute lymphoblastic leukemia

Single cell RNA sequencing of ex vivo 3D cocultures with MSCs, ECs and ALL PDXs to evaluate their interdependance Overall design: 3D ex vivo vascular-like structures(MSC-HUVEC) were co-cultured with acute lymphoblastic leukemic pateint-derived xenograft cells for 72hours

为评估各组分间的相互依存关系，本研究针对与间充质干细胞（Mesenchymal Stem Cells, MSCs）、内皮细胞（Endothelial Cells, ECs）及急性淋巴细胞白血病患者来源异种移植模型（Acute Lymphoblastic Leukemia Patient-Derived Xenografts, ALL PDXs）共培养的体外三维体系开展单细胞RNA测序。实验整体设计：将体外构建的三维类血管结构（MSC-HUVEC）与急性淋巴细胞白血病患者来源异种移植细胞共培养72小时。