Expression data from mid-third intsar Drosophila melanogaster larvae. Expression data from mid-third intsar Drosophila melanogaster larvae

Loss of Ck1alpha produces 'flyabetic' larvae that are feeding defective. In addition we found other larvae with glucose elevations show feeding aversion. We performed microarray to identify potential candidates that are involved in glucose-regulated feeding behaviors Overall design: We extracted RNA from larvae with fat body-targeted Ck1alpha loss of function by RNAi (Dcg-Gal4 X UAS-Ck1alphaRNAi) and from controls (Dcg-Gal4 X w1118) for hybridization on Affymetrix microarrays. We used 20 larvae per sample.

酪蛋白激酶1α（Ck1alpha）功能缺失会导致携带“果蝇糖尿病样”表型的幼虫出现进食缺陷。此外，我们还观察到血糖水平升高的幼虫会表现出进食厌恶行为。为筛选参与血糖调控进食行为的潜在候选基因，我们开展了基因芯片（microarray）实验。总体实验设计：我们分别从通过RNA干扰（RNAi）实现脂肪体靶向Ck1alpha功能缺失的幼虫（Dcg-Gal4 × UAS-Ck1alphaRNAi）以及对照组幼虫（Dcg-Gal4 × w1118）中提取总RNA，用于Affymetrix基因芯片杂交实验。每个样本使用20只幼虫。