microRNA expression in human tonsillar B cell populations. Homo sapiens

small RNA profiles of 6 human tonsillar B cell populatios (naive B cells, pre-germinal center B cells, centrocytes, centroblasts, memory B cells, and plasma cells) were determined by deep sequencing. These samples were compared to mouse developing lymphocytes, various hematopoietic cell lineages, and tissues. Overall design: small RNA expression profiles of 6 well defined B cell populations isolated from human tonsils.本研究通过深度测序（deep sequencing）测定了6个人类扁桃体来源B细胞群的小RNA（small RNA）表达谱，涵盖初始B细胞（naive B cells）、生发中心前B细胞（pre-germinal center B cells）、中心细胞（centrocytes）、中心母细胞（centroblasts）、记忆B细胞（memory B cells）及浆细胞（plasma cells）。将上述样本与小鼠发育淋巴细胞、各类造血细胞谱系及组织样本进行了对比分析。整体实验设计：从人类扁桃体中分离得到的6种特征明确的B细胞群的小RNA表达谱。