March 2004 surface water bacterial abundance at ten Georgia Coastal Ecosystems LTER sampling sites

Surface water samples were collected during low tide surveys near ten Georgia Coastal Ecosystem LTER sampling sites in March, 2004. Unfiltered subsamples were immediately preserved with buffered formalin and refrigerated for transport to the laboratory. The abundance of bacteria in each sample was determined using an epifluorescence microscope by counting DAPI-stained cells retained on a 0.2µm polycarbonate filter. This study was part of the GCE-LTER hydrographic monitoring program, and will be repeated quarterly.

2004年3月，研究人员在低潮期采样调查期间，于佐治亚海岸生态系统长期生态研究（Georgia Coastal Ecosystem LTER）的10个采样点位附近采集了表层水样。未过滤的子样品立即以缓冲福尔马林固定，并置于冷藏环境运至实验室。通过落射荧光显微镜（epifluorescence microscope）计数固定于0.2μm聚碳酸酯滤膜上的4',6-二脒基-2-苯基吲哚（DAPI）染色细胞，以此测定每份样品中的细菌丰度。本研究属于GCE-LTER水文监测计划的组成部分，且将按季度重复开展。