Evidence for multiple-insecticide resistance in urban Aedes albopictus populations in southern China

Aedes albopictus larvae and pupae were sampled from six sites (two sites each from urban, suburban and rural) in Guangzhou. The resistance bioassays were conducted against Bacillus thuringiensis israelensis (Bti): deltamethrin, propoxur and malathion for larvae; and deltamethrin, DDT, propoxur and malathion for adults. Mutations at the knockdown resistance (kdr) gene were analyzed, and the association between kdr mutations and phenotypic resistance was tested. (MapVEu VBP0000231)

白纹伊蚊（Aedes albopictus）的幼虫与蛹采自广州市的6个采样点位（其中城市、郊区、农村各设置2个采样点）。本研究针对该蚊虫开展抗性生物测定：幼虫阶段检测其对苏云金杆菌以色列亚种（Bacillus thuringiensis israelensis, Bti）、溴氰菊酯、残杀威以及马拉硫磷的抗性水平；成虫阶段则检测其对溴氰菊酯、滴滴涕（DDT）、残杀威以及马拉硫磷的抗性水平。此外，对击倒抗性（knockdown resistance, kdr）基因的突变位点进行了分析，并验证了kdr突变与表型抗性之间的相关性。[MapVEu VBP0000231]