Effect of MiSSP7 overexpression on the Poplar root transcriptome

The Poplar transcriptome was analyzed in Populus tremulaxPopulus alba clone 717-1B4 control roots and in two poplar lines overexpressing MiSSP7. We performed 9 hybridizations (NimbleGen) with samples derived from Populus tremulaxPopulus alba clone 717-1B4 control roots, as well as from roots of LINE1 and LINE2 MiSSP7 overexpressor poplars (3 biological replicates each). All samples were labeled with Cy3.

本研究以美洲山杨×银白杨（Populus tremulaxPopulus alba）无性系717-1B4的对照根系，以及过表达MiSSP7的LINE1、LINE2两个杨树品系的根系为材料，开展杨树转录组分析。本研究采用NimbleGen芯片完成9次杂交实验，所有样本均以Cy3荧光基团进行标记；其中对照根系组、LINE1过表达株系根系组、LINE2过表达株系根系组各设置3个生物学重复。