Homo sapiens Raw sequence reads

The key objective is to define the miRNA alterations associated with progression of advanced castration-resistant prostate cancer (CRPC) to androgen-independent neuroendocrine state. We hypothesize that this lineage switch is associated with significant alterations to the miRNAs, that in turn, drives switch in PCa gene expression patterns towards NE states. We tested our hypothesis by assessing miRNA alterations associated with transition from CRPC with adenocarcinoma features (CRPC-Adeno) to that with NE features (CRPC-NE) by performing small RNA-next generation sequencing in deidentified patient samples.

本研究的核心目标为明确与去势抵抗性前列腺癌（CRPC）进展至雄激素非依赖性神经内分泌状态相关的微小RNA（miRNA）表达改变。我们提出如下假说：该细胞谱系转换与微小RNA的显著改变存在关联，而此类改变可驱动前列腺癌的基因表达模式向神经内分泌状态转变。为验证该假说，我们对去标识化患者样本开展小RNA高通量测序，以此评估伴腺癌表型的去势抵抗性前列腺癌（CRPC-Adeno）向伴神经内分泌表型的去势抵抗性前列腺癌（CRPC-NE）转化过程中相关的微小RNA表达变化。