Comparison of miRNA profile in body fluids (plasma and bronchoalveolar fluid cytology)

The objective is to obtain miRNA representative signatures both in plasma and bronchoalveolar cell fraction that could serve as biomarker in respiratory diseases. The identification of new less invasive biomarkers is necessary to improve the detection and prognostic outcome of respiratory pathological processes. The measurement of miRNA expression through less invasive techniques such as plasma and serum have been suggested to analysis of several lung malignancies including lung cancer. These studies are assuming a common deregulated miRNA expression both in blood and lung tissue. The present study aimed to obtain miRNA representative signatures both in plasma and bronchoalveolar cell fraction that could serve as biomarker in respiratory diseases. we have compared circulating plasma miRNA with the bronchoalveolar cell fraction-derived miRNA patterns from 10 patients with several lung disease using a RT-qPCR assay.

本研究旨在获取血浆与支气管肺泡细胞组分中的微核糖核酸（microRNA, miRNA）特征谱，以期作为呼吸系统疾病的生物标志物。鉴定新型微创生物标志物，对于提升呼吸系统病理进程的检测效能与预后结局至关重要。已有研究表明，可通过血浆、血清这类微创技术检测miRNA表达水平，用于分析包括肺癌在内的多种肺部恶性肿瘤。此类研究均默认血液与肺组织中存在同步失调的miRNA表达模式。本研究旨在获取血浆与支气管肺泡细胞组分中的miRNA特征谱，以期作为呼吸系统疾病的生物标志物。本研究采用实时定量聚合酶链反应（reverse transcription quantitative polymerase chain reaction, RT-qPCR）技术，对10例多种肺部疾病患者的循环血浆miRNA与支气管肺泡细胞组分来源的miRNA表达谱展开了对比分析。