Primary DC-OT-II lymphocyte co-culture cytokine secretion.

Mean (SEM) in pg/mL, n = 4.
IFNγ/LPS matured DC were pulsed for 2 hours with OVA323–339 peptide at the indicated concentrations, and cocultured with naïve OT-II CD4+ lymphocytes. Supernatants were collected at 96 hours, and analyzed by a bead-based multiplex ELISA.

本实验结果以pg/mL为单位的平均值（标准误，Standard Error of the Mean, SEM）表示，样本量n=4。将经干扰素γ（Interferon γ, IFNγ）/脂多糖（Lipopolysaccharide, LPS）成熟的树突状细胞（Dendritic Cell, DC）以指定浓度的卵清蛋白（Ovalbumin, OVA）323–339肽段脉冲致敏2小时，随后与初始型OT-II CD4+ T淋巴细胞共培养。于共培养96小时后收集上清液，采用基于磁珠的多重酶联免疫吸附试验（Enzyme-Linked Immunosorbent Assay, ELISA）进行分析。