Genome-wide expression study of human carotid atheroma. Homo sapiens

The aim of this study was to identify new biomarkers and to investigate pathways involved in the progression of human carotid atheroma. Overall design: The study was conducted from pieces of carotid endarterectomy collected in 32 hypertensive patients. The samples contained media and neo-intima without adventitia. They were paired, including for each patient one sample of the atheroma plaque (stage IV and over of the Stary classfication) containing core and shoulders of the plaque, and one sample of distant macroscopically intact tissue (stages I and II). In addition, clinical, biological and histological data were collected.

本研究旨在识别新型生物标志物，并探究参与人类颈动脉粥样硬化进展的通路。整体实验设计：本研究使用32名高血压患者的颈动脉内膜切除术标本片段开展实验。所取样本包含血管中层与新生内膜，不含外膜。样本采用配对设计：每位患者分别获取1份符合Stary分级（Stary classification）IV级及以上的粥样硬化斑块样本，该样本包含斑块核心与肩部区域；同时获取1份远处肉眼观正常的组织样本（分级为I、II级）。此外，本研究还收集了临床、生物学及组织学相关数据。