Data from: Collective forces of tumor spheroids in three-dimensional biopolymer networks

LuB25-spheroid.zip This zip-file contains a series of brightfield images through the equatorial plane of a spheroid created from 4,000 primary mesenchymal Luminal-B breast cancer cells. The spheroid is embedded in a three-dimensional 1.2mg/ml collagen gel, together with micron-sized fiducial markers. Images were taken at an interval of 10min, spanning a period of 24h in total. The pixel-size of the image series is 1.02µm. Triduc1-spheroid.zip This zip-file contains a series of brightfield images through the equatorial plane of a spheroid created from 4,000 primary mesenchymal triple-negative breast cancer cells. The spheroid is embedded in a three-dimensional 1.2mg/ml collagen gel, together with micron-sized fiducial markers. Images were taken at an interval of 10min, spanning a period of 24h in total. The pixel-size of the image series is 1.02µm. LuB25-tumoroids.zip Each subfolder within this zip-file contains a series of brightfield images through the equatorial plane of a Luminal-B breast cancer tumoroid (tumor tissue sample). The tumoroids are embedded in a three-dimensional 1.2mg/ml collagen gel, together with micron-sized fiducial markers. Images were taken at an interval of 10min, spanning a period of 24h in total. The pixel-size of the image series is 1.02µm. A172-15000-spheroids.zip This zip-file contains three subfolders, each of which represents an independent experiment (biological replication). Each subfolder contains series of brightfield images through the equatorial plane of multiple spheroids (technical replication) created from 15,000 A172 glioblastoma cancer cells. The spheroids are embedded in a three-dimensional 1.2mg/ml collagen gel, together with micron-sized fiducial markers. Images were taken at an interval of 5min, spanning a period of 12h in total. The pixel-size of the image series is 1.29µm. U87-15000-spheroids.zip This zip-file contains three subfolders, each of which represents an independent experiment (biological replication). Each subfolder contains series of brightfield images through the equatorial plane of multiple spheroids (technical replication) created from 15,000 U87 (full name: U-87 MG) glioblastoma cancer cells. The spheroids are embedded in a three-dimensional 1.2mg/ml collagen gel, together with micron-sized fiducial markers. Images were taken at an interval of 5min, spanning a period of 12h in total. The pixel-size of the image series is 1.29µm. U87-7500-spheroids.zip This zip-file contains three subfolders, each of which represents an independent experiment (biological replication). Each subfolder contains series of brightfield images through the equatorial plane of multiple spheroids (technical replication) created from 15,000 U87 (full name: U-87 MG) glioblastoma cancer cells. The spheroids are embedded in a three-dimensional 1.2mg/ml collagen gel, together with micron-sized fiducial markers. Images were taken at an interval of 5min, spanning a period of 12h in total. The pixel-size of the image series is 1.29µm.

LuB25-spheroid.zip 该压缩包包含一系列取自细胞球体（spheroid）赤道平面的明场成像（brightfield images），该细胞球体由4000个原代间质型管腔B型乳腺癌细胞（primary mesenchymal Luminal-B breast cancer cells）构建而成。此细胞球体与微米级基准标记物（fiducial markers）一同包埋于三维1.2mg/ml胶原凝胶（collagen gel）中。图像以10分钟为间隔采集，总时长24小时，图像序列的像素尺寸为1.02µm。
Triduc1-spheroid.zip 该压缩包包含一系列取自细胞球体赤道平面的明场成像，该细胞球体由4000个原代间质型三阴性乳腺癌细胞（triple-negative breast cancer cells）构建而成。此细胞球体与微米级基准标记物一同包埋于三维1.2mg/ml胶原凝胶中。图像以10分钟为间隔采集，总时长24小时，图像序列的像素尺寸为1.02µm。
LuB25-tumoroids.zip 该压缩包内的每个子文件夹均包含一系列取自管腔B型乳腺癌肿瘤类器官（tumoroids，即肿瘤组织样本）赤道平面的明场成像。此类肿瘤类器官与微米级基准标记物一同包埋于三维1.2mg/ml胶原凝胶中。图像以10分钟为间隔采集，总时长24小时，图像序列的像素尺寸为1.02µm。
A172-15000-spheroids.zip 该压缩包包含三个子文件夹，每个子文件夹对应一次独立实验（生物学重复（biological replication））。每个子文件夹内均包含多组取自细胞球体赤道平面的明场成像，这些细胞球体由15000个A172胶质母细胞瘤（glioblastoma）细胞构建而成，属于技术重复（technical replication）样本。此类细胞球体与微米级基准标记物一同包埋于三维1.2mg/ml胶原凝胶中。图像以5分钟为间隔采集，总时长12小时，图像序列的像素尺寸为1.29µm。
U87-15000-spheroids.zip 该压缩包包含三个子文件夹，每个子文件夹对应一次独立实验（生物学重复）。每个子文件夹内均包含多组取自细胞球体赤道平面的明场成像，这些细胞球体由15000个U87（全称U-87 MG）胶质母细胞瘤细胞构建而成，属于技术重复样本。此类细胞球体与微米级基准标记物一同包埋于三维1.2mg/ml胶原凝胶中。图像以5分钟为间隔采集，总时长12小时，图像序列的像素尺寸为1.29µm。
U87-7500-spheroids.zip 该压缩包包含三个子文件夹，每个子文件夹对应一次独立实验（生物学重复）。每个子文件夹内均包含多组取自细胞球体赤道平面的明场成像，这些细胞球体由15000个U87（全称U-87 MG）胶质母细胞瘤细胞构建而成，属于技术重复样本。此类细胞球体与微米级基准标记物一同包埋于三维1.2mg/ml胶原凝胶中。图像以5分钟为间隔采集，总时长12小时，图像序列的像素尺寸为1.29µm。