Defective IRE1a Pathway Caused by Abnormal PAX6 Expression Led to Specific Human Diabetes

PAX6 is an important ß cell transcription factor. We used single cell RNA sequencing (scRNA-seq) to analyze the impact of PAX6 deficiency on ß cell transcriptomes. Overall design: human pluripoten stem cells-derived WT, PAX6+/- and PAX6-/- ß cells were analyzed using scRNA-seq

PAX6是一类重要的β细胞转录因子。本研究采用单细胞RNA测序（single cell RNA sequencing，scRNA-seq）技术，分析PAX6缺陷对β细胞转录组的影响。总体实验设计如下：对人多能干细胞来源的野生型（Wild Type，WT）、PAX6杂合缺陷（PAX6+/-）及PAX6纯合缺陷（PAX6-/-）的β细胞开展单细胞RNA测序分析。