CIL:39176, Paramecium caudatum, cell by organism, eukaryotic cell, Eukaryotic Protist, Ciliated Protist. In Cell Image Library

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

采用标准戊二醛固定工艺，随后经四氧化锇后固定、乙醇脱水并包埋于环氧树脂中。使用超薄切片机制备厚度约75nm的组织切片。原始负片经爱普生（Epson）Perfection V750 Pro扫描仪扫描，所得高分辨率图像适用于定量分析。更多相关信息可查阅：(http://www5.pbrc.hawaii.edu/allen/)