Analysis of A375 and SKMEL-103 cells after treatment with with DMSO as control or BAY-850

To identify the gene expression changes in NRAS mutant cell line SKMEL-103, and BRAF mutant cell line A375 upon BAY-850 treatment, we analyzed these cell line with either control DMSO or BAY-850 treatment via RNA sequencing. Overall design: SKMEL-103 and A375 cells were plated, samples were either treated with DMSO or BAY-850 (5-micromolar for 24 hours), cells were harvested and were used to prepare the total RNA, followed by RiboRNA depletion with random priming and RNASeq Illumina Hi-Seq 2500 platform, with three biological replicates for each sample.

为明确NRAS突变细胞系SKMEL-103与BRAF突变细胞系A375经BAY-850处理后的基因表达变化，我们通过RNA测序（RNA sequencing）技术，对分别经对照二甲基亚砜（DMSO）或BAY-850处理的上述细胞系开展分析。实验整体设计：将SKMEL-103与A375细胞进行铺板培养，随后分别以二甲基亚砜（DMSO）或5微摩尔浓度的BAY-850处理24小时；收集细胞后提取总RNA，采用随机引物法完成核糖体RNA（RiboRNA）去除，随后使用Illumina HiSeq 2500平台进行RNA测序；每个样本设置3次生物学重复。