Small RNA-Seq analysis of parental (wild type) and dcl3 mutant lines of Chlamydomonas reinhardtii. Small RNA-Seq analysis of parental (wild type) and dcl3 mutant lines of Chlamydomonas reinhardtii

DCL3 appeared several times in a forward genetic screen meant to isolate genes involved in miRNA-mediated RNA silencing in Chlamydomonas reinhardtii. Because of that, and in order to define the role of DCL3 in the processing of small RNAs on a genome-wide basis, we sequence the small RNA transcriptome of dcl3 mutant and parental lines.

DCL3在旨在分离莱茵衣藻（Chlamydomonas reinhardtii）中参与miRNA介导RNA沉默的基因的正向遗传筛选中多次被筛选得到。鉴于此，为了明确DCL3在全基因组范围内小RNA加工过程中的功能，我们对dcl3突变体及其亲本株系的小RNA转录组进行了测序。