Effect of nuclear pore complex (NPC) blockage by WGA treatment on zygotic genome activation (ZGA) during zebrafish early development

To investigate the function of nuclear pore complex (NPC) in the regulation of zygotic genome activation (ZGA), we microinjected medium dosage of WGA in zebrafish embryos at 1-cell stage to block NPC function. We then performed gene expression profiling analysis using data obtained from RNA-seq of control or WGA treated embryo at comparable developmental time point (4.3 hours post fertilization (hpf) ) or develomental stage (dome). Comparative gene expression profiling analysis of RNA-seq data for zebrafish control embryo at 4.3 hpf of dome stage and WGA treated embryos at 4.3 hpf (developmental time matched) or 5 hpf (dome-like stage).

为探究核孔复合体（nuclear pore complex, NPC）在合子基因组激活（zygotic genome activation, ZGA）调控中的功能，我们于斑马鱼1细胞期胚胎中显微注射中等剂量的小麦胚芽凝集素（Wheat Germ Agglutinin, WGA）以阻断NPC的功能。随后，我们利用对照组与WGA处理组斑马鱼胚胎的RNA测序（RNA-seq）数据开展基因表达谱分析，两组胚胎的对应发育时间点为受精后4.3小时（hours post fertilization, hpf），对应发育阶段为圆顶期（dome）。本数据集针对斑马鱼对照组胚胎（4.3 hpf 或圆顶期）与WGA处理组胚胎的RNA-seq数据开展对比基因表达谱分析，其中处理组胚胎分别取自发育时间匹配的4.3 hpf样本，以及处于类圆顶期的5 hpf样本。