Pediatric BAL Samples Targeted Locus (Loci)

The purpose was to evaluate the effect of different DNA extraction methods on microbial community profiles generated using 16S pyrosequencing - this approach was applied to both a mock community of known composition and pediatric bronchoalveolar lavage samples. 16S amplicon sequences from pediatric BAL samples and mock community: 3 pediatric BAL samples (2 from CF patients, and 1 from a non-CF patient) and a simulated mock BAL sample.

本数据集旨在评估不同DNA提取方法对基于16S焦磷酸测序（16S pyrosequencing）所获得的微生物群落谱的影响。该分析方法同时应用于已知组成的模拟群落（mock community）与儿科支气管肺泡灌洗（bronchoalveolar lavage, BAL）样本。本次研究涉及的16S扩增子序列来自儿科BAL样本与模拟群落：3份儿科BAL样本（其中2份来自囊性纤维化（CF）患者，1份来自非CF患者）以及1份模拟BAL样本。