ExtFig3A_RACE_TUBA1B_R3_LG293_annotated.tiff

Xrn1 knock out A549 cells were infected with WT PR8 or PR8-PA(∆X), or mock infected. 5’ RACE was then performed using primers specific for BCAP31, TUBA1B, INSIG1 or SLC7A5, positioned ~ 200-300 nucleotides downstream of the predicted cut sites.The PCR products were run on an agarose gel.

将Xrn1基因敲除的A549细胞分别用野生型PR8毒株、PR8-PA(ΔX)毒株进行感染，同时设置空白感染对照组。随后以针对BCAP31、TUBA1B、INSIG1及SLC7A5基因的特异性引物开展5' 末端快速扩增技术（5'-Rapid Amplification of cDNA Ends，5' RACE）实验，该引物位于各靶基因预测剪切位点下游约200~300个核苷酸处。最后将所得PCR产物经琼脂糖凝胶电泳进行检测。