[RAW VALIDATED DATA] Properties of seawater and particulate matter (fluorescence) from a WETLabs Eco-FL sensor mounted on the continuous surface water sampling system during the Tara Oceans expedition 2009-2013

The Tara Oceans Expedition (2009-2013) sampled the world oceans on board a 36 m long schooner, collecting environmental data and organisms from viruses to planktonic metazoans for later analyses using modern sequencing and state-of-the-art imaging technologies. Tara Oceans Data are particularly suited to study the genetic, morphological and functional diversity of plankton. The present data set provides raw validated data of continuous measurements made with a WETLabs Eco-FL sensor mounted on the flowthrough system between June 4th, 2011 and March 30th, 2012. Data was recorded approximately every 10s. Two issues affected the data: 1. Periods when the water 0.2µm filtered water were used as blanks and 2. Periods where fluorescence was affected by non-photochemical quenching (NPQ, chlorophyll fluorescence is reduced when cells are exposed to light, e.g. Falkowski and Raven, 1997). Median data and their standard deviation were binned to 5min bins with period of light/dark indicated by an added variable (so that NPQ affected data could be neglected if the user so chooses). Data was first calibrated using HPLC data collected on the Tara (there were 36 data within 30min of each other). Fewer were available when there was no evident NPQ and the resulting scale factor was 0.0106 mg Chl m-3/count. To increase the calibration match-ups we used the AC-S data which provided a robust estimate of Chlorophyll (e.g. Boss et al., 2013). Scale factor computed over a much larger range of values than HPLC was 0.0088 mg Chl m-3/count (compared to 0.0079 mg Chl m-3/count based on manufacturer). In the archived data the fluorometer data is merged with the TSG, raw data is provided as well as manufacturer calibration constants, blank computed from filtered measurements and chlorophyll calibrated using the AC-S. For a full description of the processing of the Eco-FL please see Taillandier (2015).

塔拉海洋科考航次（2009-2013年）依托一艘36米长的纵帆船对全球海洋开展采样工作，收集了从病毒到浮游后生动物的各类生物样本与环境数据，以供后续借助现代测序技术与最先进的成像技术开展分析研究。塔拉海洋数据集尤其适用于探究浮游生物的遗传、形态与功能多样性。本数据集提供了2011年6月4日至2012年3月30日期间，搭载于流通系统的WETLabs Eco-FL传感器所采集的经过验证的原始连续测量数据。数据采集间隔约为10秒。本次数据存在两处影响因素：1. 以0.2µm过滤海水作为空白对照的时段；2. 荧光受非光化学淬灭（Non-Photochemical Quenching，NPQ，即细胞受光照时叶绿素荧光会降低，参见Falkowski和Raven，1997年）影响的时段。研究人员将中位数数据及其标准差整合为5分钟时段的分箱数据，并通过新增变量标注光照/黑暗时段，以便使用者可根据需求剔除受NPQ影响的数据。数据最初依托塔拉科考航次采集的高效液相色谱（High Performance Liquid Chromatography, HPLC）数据进行校准，每30分钟内存在36组匹配数据。当无明显NPQ影响时，可用的匹配数据较少，最终得到的缩放因子为0.0106 mg Chl m⁻³/计数。为扩充校准匹配数据集，我们采用了可稳健估算叶绿素浓度的AC-S数据（参见Boss等人，2013年）。基于远大于HPLC数据范围的数值区间计算得到的缩放因子为0.0088 mg Chl m⁻³/计数，而厂商提供的缩放因子为0.0079 mg Chl m⁻³/计数。在归档数据中，荧光计数据已与温盐仪（Temperature-Salinity-Gauge, TSG）数据合并；数据集同时提供原始数据、厂商校准常数、基于过滤海水测量得到的空白值，以及通过AC-S校准得到的叶绿素浓度数据。如需了解Eco-FL传感器数据处理的完整细节，请参阅Taillandier（2015）的相关研究。