Medicago truncatula strain:Jemalong A17/J5 Transcriptome or Gene expression. Medicago truncatula strain:Jemalong A17/J5

This experiment was part of a larger study to elucidate the early effects of Nod factor treatment on the Medicago truncatula phosphoproteome, transcriptome and metabolome in wildtype and two mutant strains. In this experiment, RNA-Seq was used to measure transcriptional changes in plant roots treated +/- Nod factors for 1 hr. RNA was extracted and sequenced from three biological replicates of each treatment condition in wildtype as well as mutants in nfp and dmi3, two genes known to be involved in the response pathway.

本实验隶属于一项更大规模的研究，旨在阐明结瘤因子（Nod factor）处理对野生型及两种突变株系蒺藜苜蓿（Medicago truncatula）的磷酸化蛋白质组、转录组与代谢组的早期影响。本实验采用RNA测序（RNA-Seq）技术，对施加与未施加结瘤因子处理1小时的植物根系的转录变化进行检测。研究针对野生型，以及nfp、dmi3两个已知参与该结瘤响应通路的基因的突变体，为每个处理条件设置了三个生物学重复，分别提取RNA并完成测序。