Panels used for flow cytometry.

Displayed are the combinations of conjugated monoclonal antibodies (mAb) against specific antigens within each panel. In addition, the clone for each specific mAb is shown. Each panel was used for flow cytometric (FCM) analysis of bone marrow, cord blood, peripheral blood, inguinal LN, liver LN, and spleen samples of human donors (all n = 5). Thawed MNC fractions of the human tissue samples were assessed on a NaviosTM 10-color flow cytometer and analyzed using Kaluza Software® 1.0 (Beckman coulter). Panel 1 was used to identify different NK cell developmental stages based on CD34, CD117, CD94 and CD56 expression profiles.10 Additionally, expression of early development markers CD133 and CD33, stimulatory co-receptor 2B4 (CD244), and C-type lectin NKG2A were analyzed to refine the definition of the different NK cell developmental stages. Panel 2 and 3 were used to analyze the NK cell receptor repertoire of CD45+CD56brightCD16+/−CD3− and CD45+CD56dimCD16+CD3− NK cells consisting of inhibitory and stimulatory receptors. Inhibitory receptors contain KIR (CD158a, CD158b, CD158e1) and NKG2A (CD159a). Stimulatory receptors contain NCR (CD335/336/337), NKG2C (CD159c), NKG2D (CD314), and 2B4 (CD244).

本数据集展示了各检测组合（panel）中针对特定抗原的荧光标记单克隆抗体（monoclonal antibody, mAb）的组合方案，同时标注了每种特异性单克隆抗体的克隆号。
每组检测组合均用于对5名人类供者的骨髓、脐带血、外周血、腹股沟淋巴结（inguinal LN）、肝脏淋巴结（liver LN）及脾脏样本开展流式细胞术（flow cytometric, FCM）分析，所有样本的受试人数均为n=5。
本研究对解冻后的人类组织样本单个核细胞（mononuclear cell, MNC）组分，采用NaviosTM 10色流式细胞仪进行检测，并使用Kaluza Software® 1.0（贝克曼库尔特（Beckman Coulter））完成数据分析。
检测组合1用于基于CD34、CD117、CD94及CD56的表达谱鉴定不同的自然杀伤细胞（Natural Killer cell, NK）发育阶段[10]。此外，通过分析早期发育标志物CD133、CD33、刺激性共受体2B4（CD244）以及C型凝集素NKG2A的表达，进一步细化了不同NK细胞发育阶段的判定标准。
检测组合2与3则用于分析CD45+CD56brightCD16+/−CD3−及CD45+CD56dimCD16+CD3− NK细胞的NK细胞受体库，该受体库包含抑制性与刺激性两类受体。其中抑制性受体包括杀伤细胞免疫球蛋白样受体（Killer Immunoglobulin-like Receptor, KIR）（CD158a、CD158b、CD158e1）以及NKG2A（CD159a）；刺激性受体则包括自然细胞毒性受体（Natural Cytotoxicity Receptor, NCR）（CD335/336/337）、NKG2C（CD159c）、NKG2D（CD314）以及2B4（CD244）。