Comparative transcriptomic analysis between a high and a low sulfite producers wine yeast strains. Saccharomyces cerevisiae

The goal of this study was to compare the expression level of the whole genome of two wine yeast strains highly differing in their sulfite production (High producer strain: 10281A; Low producer strain: 1764A). Conditions maximizing SO2 production were selected: nitrogen rich media (425 mg/l assimilable nitrogen) and low temperature (16°C). This transcriptomic analysis was performed during the sulfite production phase, just after the entry in stationary phase. This analysis is part of a global work, aiming at the identification of the molecular basis of sulfite production by wine yeasts through physiologic, transcriptomic and genetic studies. Overall design: Two strains compared in the same conditions with biological replicates following a dye-swap design.

本研究旨在比较两株亚硫酸盐生成能力差异显著的葡萄酒酵母菌株的全基因组表达水平：高产量菌株10281A与低产量菌株1764A。本研究选取了最大化亚硫酸盐（SO₂）生成的培养条件：富氮培养基（可同化氮（assimilable nitrogen）含量为425 mg/L）与低温（16℃）环境。该转录组学分析（transcriptomic analysis）在亚硫酸盐生成阶段，即菌株进入稳定期后即刻开展。本分析隶属于一项系统性研究工作，该工作旨在通过生理学、转录组学与遗传学研究，解析葡萄酒酵母亚硫酸盐生成的分子机制。实验整体设计：将两株菌株在相同培养条件下进行对比，并采用染料互换（dye-swap）设计设置生物学重复。