Gene expression profiling of Barrett’s esophageal tissues and esophageal adenocarcinoma specimens

Specimens were collected from esophageal adenocarcinoma patients undergoing esophagectomy for adenocarcinoma at the University of Michigan Health System between 1991 and 2004. Written consent was obtained from each patient according to the approval and guidelines of the University of Michigan institutional review board. Patients receiving treatment with chemotherapy and/or radiotherapy prior to surgery were excluded. Tissue samples were fresh-frozen in liquid nitrogen and stored at −80 °C until use. Cellularity of metaplastic, dysplastic, and tumor samples were assured to be greater than 70% before sample RNA was isolated. RNA from 31 Barrett’s and 15 adenocarcinoma samples was extracted and processed for hybridization on Affymetrix HG-U133A microarrays.

本数据集的样本采集自1991年至2004年间，取自密歇根大学医疗体系（University of Michigan Health System）内因腺癌接受食管切除术的食管腺癌患者。所有患者均已签署书面知情同意书，相关研究流程符合密歇根大学机构审查委员会（Institutional Review Board）的审批要求与指南规范。术前接受过化疗和/或放疗的患者被排除出本数据集。组织样本经液氮速冻后，于-80℃环境下保存直至实验使用。在提取样本RNA前，已确保化生样本、异型增生样本及肿瘤样本的肿瘤细胞占比均高于70%。研究人员从31例Barrett食管样本及15例食管腺癌样本中提取RNA，并将其处理后用于Affymetrix HG-U133A基因芯片的杂交实验。