Single cell transcriptomics of human brain epileptic lesions identifies pro-inflammatory immune mechanisms based on interlacing cellular immune networks. Single cell transcriptomics of human brain epileptic lesions identifies pro-inflammatory immune mechanisms based on interlacing cellular immune networks

We analysed 85780 single cells from epileptic brain tissues using Cellular Indexing of Transcriptomes and Epitopes by Sequencing (CITE-seq) to dissect the architecture of the immuno-transcriptome of the diseased epileptic human brain. dissect the architecture of the immuno-transcriptome of the diseased epileptic human brain. Our approach uncovered in all patients, well organized pro-inflammatory microenvironment pivoting on activated, pro-inflammatory microglia in a closely-knit network where resident cells attract and manipulate in a pro-inflammatory fashion infiltrating innate and adaptive immune cells. These findings were corroborated by ligand-receptor interactome analysis, which demonstrated potential mechanisms of infiltration and by evidence of direct interactions between microglia and T cells likely contributing to pathogenesis. Overall design: Immune cells Isolated from human brain tissue from epilepsy Patients analyzed ---------------------------------------------------------------- Authors state "We are not allowed to deposit Human Patients sequence data to archives."

本研究采用测序介导的转录组与表位细胞索引技术（Cellular Indexing of Transcriptomes and Epitopes by Sequencing, CITE-seq），对85780个来自癫痫脑组织的单细胞进行分析，以解析癫痫病变人脑的免疫转录组架构。本研究方法在所有受试患者中均发现了以活化促炎性小胶质细胞为核心的高度组织化促炎微环境：该微环境构成紧密网络，驻留细胞以促炎表型招募并调控浸润的固有免疫与适应性免疫细胞。上述研究结果通过配体-受体相互作用组分析得到验证，该分析揭示了免疫细胞浸润的潜在机制；同时存在小胶质细胞与T细胞直接相互作用的相关证据，这类相互作用可能参与疾病的致病过程。实验整体设计：从癫痫患者的人脑组织中分离免疫细胞并开展相关分析。作者声明："我们无法将人类患者的测序数据提交至公共数据库。"