RNA-seq analysis of Salmonella enterica Typhimurium LT2

We performed whole-genome transcriptomic analyses of the Salmonella Typhimimurium genome during glucose-phosphate stress. In particular, we wanted to elucidate the role of the the small RNA SgrS and protein regulator SgrT in the stress response. Overall design: Wild-type Salmonella cells, mutants of Salmonella either lacking sgrS or lacking SgrS RNA/ SgrT peptide function were subject to glucose-phoshate stress

本研究对处于葡萄糖-磷酸胁迫（glucose-phosphate stress）条件下的鼠伤寒沙门氏菌（Salmonella Typhimurium）全基因组开展了转录组分析。具体而言，本研究旨在阐明小RNA（small RNA）SgrS与蛋白质调节因子SgrT（protein regulator SgrT）在该胁迫应答过程中的作用。
实验设计概述：将野生型沙门氏菌细胞，以及缺失sgrS基因或丧失SgrS RNA/SgrT肽功能的沙门氏菌突变株，均置于葡萄糖-磷酸胁迫环境中进行处理。