Responses of fully proliferating Arabidopsis leaves to short-term osmotic stress

Drought is an important environmental factor affecting plant growth and biomass production. Despite this importance little is known on the molecular mechanisms regulating plant growth under water limiting conditions. The main goal of this work was to investigate, using a combination of growth and molecular profiling techniques, how stress arrests CELl proliferation in Arabidopsis thaliana leaves upon osmotic stress imposition. Plants were grown on nylon meshes overlaying control 0.5MS media. At 9 DAS when third leaf is fully proliferating seedlings were transfered to either mannitol (25mM) or control plates, and proliferating leaves were micro-dissected at 1.5h, 3h, 12h and 24h after stress imposition and using RNAlater solution (25mM). Samples were from three independent biological experiments. Each sample was pooled from multiple plants and multiple plates in one experiment. RNA samples were submitted to ATH1 array hybridization.

干旱是影响植物生长与生物量积累的关键环境胁迫因子。尽管其重要性已得到广泛认可，但目前学界对水分限制条件下调控植物生长的分子机制仍缺乏深入认知。本研究旨在结合生长分析与分子谱分析技术，探究渗透胁迫施加后，胁迫如何抑制拟南芥（Arabidopsis thaliana）叶片的细胞增殖。实验植株种植于覆盖对照0.5×MS培养基的尼龙网载体上。播种后第9天（9 DAS, Days After Sowing），当第三片叶完全处于细胞增殖阶段时，将幼苗转移至含25mM甘露醇的胁迫培养基平板或对照培养基平板中；随后分别于胁迫施加后1.5h、3h、12h及24h，采用RNAlater溶液（RNAlater）固定并显微切割处于增殖状态的叶片。本研究样本来自3次独立生物学重复实验，单次实验中每份样本均混合自多株植株及多块培养平板的取材材料。提取的RNA样本均用于ATH1基因芯片杂交实验。