Gnotobiotic mouse cecal epithelium; B.thetaiotaomicron-B. longum mono- versus bi-association, PR chow, NMRI

Laser captured cecal epithelium from gnotobiotic mice colonized with B. thetaiotaomicron or B. longum (mono-association) or B. thetaiotaomicron bi-association with B. longum. Samples were derived from 10d colonizations of NMRI mice fed a standard-chow polysaccharide rich (PR) diet. Keywords: Germ-free vs. Mono-associations or Bi-association, in vivo Total RNA was prepared from laser captured cecal epithelium of 10 day associated gnotobiotic mice, double amplified, and hybridized to 430 2.0 GeneChips or to GlycoGeneChipsv1.

本数据集的样本为激光捕获的盲肠上皮细胞，供体为悉生小鼠（gnotobiotic mice）：这些小鼠分别定植多形拟杆菌（Bacteroides thetaiotaomicron）或长双歧杆菌（Bifidobacterium longum）以实现单定植（mono-association），或同时定植二者以实现共定植（bi-association）。所有样本均取自喂食富含多糖（polysaccharide rich，缩写PR）的标准饲料的NMRI小鼠，经10天定植后获取。关键词：无菌状态（Germ-free）与单定植、共定植对比，体内（in vivo）。从上述经10天定植的悉生小鼠的激光捕获盲肠上皮中提取总RNA，经两轮扩增后，分别与430 2.0基因芯片（GeneChip）或糖基化基因芯片v1（GlycoGeneChipsv1）进行杂交。