Zooplankton Count Dataset

Zooplankton were enumerated under a dissection microscope using a gridded square petri dish. Samples with large numbers of zooplankton were diluted to a known volume (i.e., 300 ml) and at minimum 4 subsamples were taken using a 10 ml Hensen-Stempel sampler following agitation. Subsample counts were adjusted to reflect the full 20 l sample volume, and averaged to obtain 1 value for that date, location, and sampling method combination. During lab work, the Bubbly Creek sample from August 22^nd was lost after one 10 ml subsample was counted (beaker broke), we maintained this sample in the analysis despite lower confidence in community composition (78 individuals counted compared to mean of 124, median of 54, and standard deviation of 176 across 146 samples). Zooplankton were classified into the following groups: Bosminidae, Calanoida, Chironomid larvae, Cyclopoida, Chydoridae, Daphniidae, Diaphanosoma, Moinidae, Naididae, Ostracoda, <i>Polyphemus pediculus</i>, and the presence of nauplii and rotifers were noted but not counted.

研究人员使用带网格的方形培养皿，在解剖显微镜下对浮游动物进行计数。对于浮游动物丰度较高的样品，将其稀释至固定体积（即300毫升）；振荡混匀后，采用10毫升亨森-施坦佩尔采样器（Hensen-Stempel sampler）至少采集4份子样品。将子样品的计数结果换算至完整20升的原始样品体积，随后取平均值，得到对应采样日期、地点与采样方法组合下的唯一统计值。实验过程中，8月22日采集的气泡溪（Bubbly Creek）样品在完成1份10毫升子样品计数后因烧杯破碎受损丢失，尽管该样品的群落组成可信度较低（已计数个体仅78只，而146份样品的均值为124只、中位数为54只、标准差为176），研究人员仍将其纳入后续分析。浮游动物被划分为以下类群：象鼻蚤科（Bosminidae）、哲水蚤目（Calanoida）、摇蚊幼虫（Chironomid larvae）、剑水蚤目（Cyclopoida）、盘肠蚤科（Chydoridae）、溞科（Daphniidae）、透明溞属（Diaphanosoma）、粗毛蚤科（Moinidae）、颤蚓科（Naididae）、介形纲（Ostracoda）、独眼溞（Polyphemus pediculus）；其中无节幼体与轮虫仅记录其存在与否，不进行计数。